

Project Research
Functional foods (also known as ' nutraceutical', 'super' or 'medicinal') are foods or isolated food ingredients that provide a positive physiological effect on consumers health beyond just basic nutritional value. Functional foods can come from various sources such as naturally derived, fortification, enrichment or just have functional ingredients.
Foods containing probiotics, are single or mixed culture of organisms that beneficially contribute by improving the microbial flora in the gut. Many probiotic foods are associated with dairy, especially here in Ireland. With this in mind people who are in need of probiotic inclusion in their diets to improve their gut health and deal with gastrointestinal disorders such as Inflammatory Bowel Syndrome and Inflammatory Bowel Disease may not be able to consume dairy as part of their diet, whether by choice such as veganism or as a matter of being lactose intolerant, allergic to dairy products or even if subject to a diet that requires them to consume a low cholesterol diet, as a consequence there is a niche for non-dairy probiotic products.
Some 10-15% of Europeans are believed to be lactose intolerant and parts of Africa and Asia as high as 80+% lactose intolerance levels where they would not be accustomed to dairy consumption.
Functional Foods
Kefir
There are two varieties of kefir grains: water kefir and milk kefir. Kefir creates ideal conditions in the digestive tract for the colonization of friendly bacteria. Made up of a polysaccharide complex containing a bacteria, yeast, enzyme mixture which incorporate these beneficial microorganisms to make a cultured product. The grains are removed from the liquid product and can be reused to make more kefir culture product.
Because kefir is such a balanced and nourishing food, it contributes to a healthy immune system, it cleanses the intestines, provides beneficial bacteria and yeast, vitamins and minerals, and complete proteins. In addition contains minerals and essential amino acids that help the body with healing and maintenance functions. Regular use can help relieve intestinal disorders, promote bowel movement, reduce flatulence and create a healthier digestive system.
There are a lot of differences between water kefir and milk kefir. Aside from being cheaper (since you only need water and sugar to prepare it), water kefir is also dairy-free, making it a perfect choice for those who want to avoid dairy. Water kefir is a lighter drink, making it easier to drink in larger quantities especially during warm weather for hydration.
Equipment materials
Once we had experimented with home fermentation and confirmed that it does in fact work to produce a cultured water product we needed to research on how to upscale from this. We researched between three different materials in Glass, Steel and Plastic. Here is where we had conflicting opinions on the direction to take the bioprocess, whether we should use glass or steel. We researched all the pros and cons of each and came up with a compromise utilizing the best aspects of glass and steel materials.
Pro's
Con's
Glass
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Cheap when bought in bulk ($1per 10L container)
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Chemically inert
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Easily cleaned & replaced
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Simplicity in set up and management
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Fail safe with numbers, if one fermentation jar fails whole batch is not ruined
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Transparent
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Eco Friendly
Steel
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Robust
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Versatile
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Large Scale
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Automated
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Homogenisation
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Flavourings and additives can easily be added into product
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Auxiliaries for monitoring
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Can be bought second hand
Plastic
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Cheap
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Durable
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Relatively easy to clean
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Lightweight
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Transparent
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Glass
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Heavy & fragile
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Not Automated, needs to be manually monitored and handled
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Difficult to extract product and transfer into vessel for sale distribution
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Too expensive to design large customised container, numerous small containers would be more practical
Steel
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More expensive than other containers
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If something goes wrong the whole batch is lost
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Metal interacts with the kefir grains hindering productivity (Stainless steel can be used safely but still affects productivity)
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Requires constant maintenance (Maintenance costs)
Plastic
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Not chemically inert , interacts with kefir grains
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Can develop scratches which make it more difficult to sanitize and clean
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Need replacing over time
Home Fermentation
To make sure that the kefir grains do in fact produce a cultured product we tested out by fermenting the grains ourselves at home.
Venturi, A., Gionchetti, P., Rizzello, F., Johansson, R., Zucconi, E., Brigidi, P., Matteuzzi, D. and Campieri, M., 1999. Impact on the composition of the faecal ora by a new probiotic preparation: preliminary data on maintenance treatment of patients with ulcerative colitis. Aliment Pharmacol Ther, 13(8), p.1103-1108.
Composition of Water Kefir Grains: Bacteria and Yeasts. 2015. Composition of Water Kefir Grains: Bacteria and Yeasts. [ONLINE] Available at: www.culturesforhealth.com/water-kefir-grains-composition-bacteria-yeast. [Accessed 10 December 2015].
Lopez-Varela, S., Gonzalez-Gross, M. and Marcos, A., 2002. Functional foods and the immune system: a review. European journal of clinical nutrition, 56, pp.S29-33.
Health Benefits of Probiotics: A Review. 2015. Health Benefits of Probiotics: A Review. [ONLINE] Available at: http://www.hindawi.com/journals/isrn/2013/481651/. [Accessed 10 December 2015].
Probiotics
Probiotics refer to bacteria that are live microorganisms that are consumed, which provide a health benefit when consumed. Through competitive exclusion they line the intestines and reduce the effect of GI diseases. Some Lactic Acid Bacteria (LAB) have the ability to reduce inflammatory reactions in the body and can also act as antimicrobials which will promote a healthy environment in the gut. For a product to be probiotic it must contain at least 10 to the power of -6 CFU per gram, this amount will insure that the bacteria can have a beneficial effect. Probiotics are a strong candidate for inclusion in a functional food because of the evidence to show the treatment and prevention of intestinal disorders.